Translational_Unit
RBS+eGFP+T

Part:BBa_K193205

Designed by: Nao Nakatani   Group: iGEM09_Tokyo_Tech   (2009-10-14)

TmTHP with EGFP for fusion of Anti-Freeze Protein and RBS

TmTHP with EGFP for fusion of Anti-Freeze Protein and RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1016
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1016
    Illegal NheI site found at 1034
    Illegal NotI site found at 1046
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1016
    Illegal BamHI site found at 551
    Illegal XhoI site found at 452
    Illegal XhoI site found at 1055
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1016
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1016
    Illegal AgeI site found at 761
    Illegal AgeI site found at 800
    Illegal AgeI site found at 872
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aTmTHP with EGFP for fusion of Anti-Freeze Protein and RBS